To establish endometriosis in the nude mouse, endometrial tissues were maintained in 1 nm estradiol (E) for 24 h and subsequently injected into ovariectomized nude mice. Proliferative phase human endometrial biopsies were obtained from healthy donors and established as organ cultures or used to isolate ES cells. The aim was to evaluate effects of simvastatin on development of endometriosis in a nude mouse model. Recent in vitro studies have shown that statins decrease proliferation of endometrial stroma (ES) and inhibit angiogenesis. Simvastatin protects against the development of endometriosis in a nude mouse model.īruner-Tran, Kaylon L Osteen, Kevin G Duleba, Antoni JĮndometriosis is a common condition associated with infertility and pelvic pain in women. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors. CONCLUSION: OB glue paste technique is easy to follow. The occurrence of ascites was 47.80% and 36.96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. RESULTS: The success rates of orthotopic transplan-tation of the two models were 94.20% and 96%. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed. METHODS: Using OB glue paste technique, orthotopic transplantation models were established by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Shi, Jun Wei, Pin-Kang Zhang, Shen Qin, Zhi-Feng Li, Jun Sun, Da-Zhi Xiao, Yan Yu, Zhi-Hong Lin, Hui-Ming Zheng, Guo-Jing Su, Xiao-Mei Chen, Ya-Lin Liu, Yan-Fang Xu, LingĪIM: To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique.
OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models
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